Select Publications
The intersection between stress responses and inositol pyrophosphates in Saccharomyces cerevisiae. Morrissette, V.A.#, and R.J. Rolfes (2020) Curr. Genetics 66, 901-910. doi: 10.1007/s00294-020-01078-8. Epub 2020 Apr 23.PMID: 32322930
The InsP7 phosphatase Siw14 controls levels of inositol pyrophosphates that are necessary for the environmental stress response of Saccharomyces cerevisiae. Steidle, E.A.#, V.A. Morrissette#, K. Fujimaki, L. Chong, A.C. Resnick, A.P. Capaldi, and R.J. Rolfes (2020) J. Biol. Chem. 295, 2043-2056. PMCID: PMC7029108 (available on 2021-02-14), DOI: 10.1074/jbc.RA119.012148
# means co-first authors
Functional Mapping of Transcription Factor Grf10 That Regulates Adenine-Responsive and Filamentation Genes in Candida albicans. Wangsanut T, Tobin JM, Rolfes RJ. mSphere. 2018 Oct 24;3(5). pii: e00467-18. doi: 10.1128/mSphere.00467-18. Erratum in: mSphere. 2019 Feb 20;4(1):. PMID: 30355670 Free PMC Article
Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates. Wang H, Gu C, Rolfes RJ, Jessen HJ, Shears SB. J Biol Chem. 2018 May 4;293(18):6905-6914. doi: 10.1074/jbc.RA117.001670. Epub 2018 Mar 14. PMID: 29540476 Free PMC Article
Grf10 and Bas1 Regulate Transcription of Adenylate and One-Carbon Biosynthesis Genes and Affect Virulence in the Human Fungal Pathogen Candida albicans. Wangsanut T, Ghosh AK, Metzger PG, Fonzi WA, Rolfes RJ. mSphere. 2017 Aug 2;2(4). pii: e00161-17. doi: 10.1128/mSphere.00161-17. eCollection 2017 Jul-Aug. PMID: 28776040 Free PMC Article
Identification of Genes in Candida glabrata Conferring Altered Responses to Caspofungin, a Cell Wall Synthesis Inhibitor.
Rosenwald AG, Arora G, Ferrandino R, Gerace EL, Mohammednetej M, Nosair W, Rattila S, Subic AZ, Rolfes R.
G3 (Bethesda). 2016 Sep 8;6(9):2893-907. doi: 10.1534/g3.116.032490.
PMID: 27449515 Free PMC Article
A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the β-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5). Steidle EA, Chong LS, Wu M, Crooke E, Feidler D, Resnick AC, Rolfes RJ.
J Biol Chem. 2016 Mar 25;291(13):6772-83. doi: 10.1074/jbc.M116.714907. Epub 2016 Jan 31.
PMID: 26828065 Free PMC Article
The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans.Ghosh AK, Wangsanut T, Fonzi WA, Rolfes RJ.
FEMS Yeast Res. 2015 Dec;15(8). pii: fov093. doi: 10.1093/femsyr/fov093. Epub 2015 Oct 15.
PMID: 26472755 Free PMC Article
Using electrospun poly(ethylene-oxide) nanofibers for improved retention and efficacy of bacteriolytic antibiotics.
Gatti JW, Smithgall MC, Paranjape SM, Rolfes RJ, Paranjape M.
Biomed Microdevices. 2013 Oct;15(5):887-93. doi: 10.1007/s10544-013-9777-5.
PMID: 23764950 [PubMed - in process]
Candida albicans isolates from the gut of critically ill patients respond to phosphate limitation by expressing filaments and a lethal phenotype.
Romanowski K, Zaborin A, Valuckaite V, Rolfes RJ, Babrowski T, Bethel C, Olivas A, Zaborina O, Alverdy JC.
PLoS One. 2012;7(1):e30119. doi: 10.1371/journal.pone.0030119. Epub 2012 Jan 13.
PMID: 22253901 [PubMed - indexed for MEDLINE] Free PMC Article
Goa1p of Candida albicans localizes to the mitochondria during stress and is required for mitochondrial function and virulence.
Bambach A, Fernandes MP, Ghosh A, Kruppa M, Alex D, Li D, Fonzi WA, Chauhan N, Sun N, Agrellos OA, Vercesi AE, Rolfes RJ, Calderone R.
Eukaryot Cell. 2009 Nov;8(11):1706-20. doi: 10.1128/EC.00066-09. Epub 2009 Aug 28.
PMID: 19717740 [PubMed - indexed for MEDLINE] Free PMC Article
Activation of the ADE genes requires the chromatin remodeling complexes SAGA and SWI/SNF.
Koehler RN, Rachfall N, Rolfes RJ.
Eukaryot Cell. 2007 Aug;6(8):1474-85. Epub 2007 Jun 15.
PMID: 17573544 [PubMed - indexed for MEDLINE] Free PMC Article
Regulation of purine nucleotide biosynthesis: in yeast and beyond.
Rolfes RJ.
Biochem Soc Trans. 2006 Nov;34(Pt 5):786-90.
PMID: 17052198 [PubMed - indexed for MEDLINE]
DNA-bound Bas1 recruits Pho2 to activate ADE genes in Saccharomyces cerevisiae.
Som I, Mitsch RN, Urbanowski JL, Rolfes RJ.
Eukaryot Cell. 2005 Oct;4(10):1725-35.
PMID: 16215179 [PubMed - indexed for MEDLINE] Free PMC Article
Mutations in the Pho2 (Bas2) transcription factor that differentially affect activation with its partner proteins Bas1, Pho4, and Swi5.
Bhoite LT, Allen JM, Garcia E, Thomas LR, Gregory ID, Voth WP, Whelihan K, Rolfes RJ, Stillman DJ.
J Biol Chem. 2002 Oct 4;277(40):37612-8. Epub 2002 Jul 26.PMID: 12145299 [PubMed - indexed for MEDLINE] Free Article
Functional mapping of Bas2. Identification of activation and Bas1-interaction domains.
Hannum C, Kulaeva OI, Sun H, Urbanowski JL, Wendus A, Stillman DJ, Rolfes RJ.
J Biol Chem. 2002 Sep 13;277(37):34003-9. Epub 2002 Jul 10.
PMID: 12110691 [PubMed - indexed for MEDLINE] Free Article
ade9 is an allele of SER1 and plays an indirect role in purine biosynthesis.
Buc PS, Rolfes RJ.
Yeast. 1999 Sep 30;15(13):1347-55.
PMID: 10509016 [PubMed - indexed for MEDLINE]
The intersection between stress responses and inositol pyrophosphates in Saccharomyces cerevisiae. Morrissette, V.A.#, and R.J. Rolfes (2020) Curr. Genetics 66, 901-910. doi: 10.1007/s00294-020-01078-8. Epub 2020 Apr 23.PMID: 32322930
The InsP7 phosphatase Siw14 controls levels of inositol pyrophosphates that are necessary for the environmental stress response of Saccharomyces cerevisiae. Steidle, E.A.#, V.A. Morrissette#, K. Fujimaki, L. Chong, A.C. Resnick, A.P. Capaldi, and R.J. Rolfes (2020) J. Biol. Chem. 295, 2043-2056. PMCID: PMC7029108 (available on 2021-02-14), DOI: 10.1074/jbc.RA119.012148
# means co-first authors
Functional Mapping of Transcription Factor Grf10 That Regulates Adenine-Responsive and Filamentation Genes in Candida albicans. Wangsanut T, Tobin JM, Rolfes RJ. mSphere. 2018 Oct 24;3(5). pii: e00467-18. doi: 10.1128/mSphere.00467-18. Erratum in: mSphere. 2019 Feb 20;4(1):. PMID: 30355670 Free PMC Article
Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates. Wang H, Gu C, Rolfes RJ, Jessen HJ, Shears SB. J Biol Chem. 2018 May 4;293(18):6905-6914. doi: 10.1074/jbc.RA117.001670. Epub 2018 Mar 14. PMID: 29540476 Free PMC Article
Grf10 and Bas1 Regulate Transcription of Adenylate and One-Carbon Biosynthesis Genes and Affect Virulence in the Human Fungal Pathogen Candida albicans. Wangsanut T, Ghosh AK, Metzger PG, Fonzi WA, Rolfes RJ. mSphere. 2017 Aug 2;2(4). pii: e00161-17. doi: 10.1128/mSphere.00161-17. eCollection 2017 Jul-Aug. PMID: 28776040 Free PMC Article
Identification of Genes in Candida glabrata Conferring Altered Responses to Caspofungin, a Cell Wall Synthesis Inhibitor.
Rosenwald AG, Arora G, Ferrandino R, Gerace EL, Mohammednetej M, Nosair W, Rattila S, Subic AZ, Rolfes R.
G3 (Bethesda). 2016 Sep 8;6(9):2893-907. doi: 10.1534/g3.116.032490.
PMID: 27449515 Free PMC Article
A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the β-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5). Steidle EA, Chong LS, Wu M, Crooke E, Feidler D, Resnick AC, Rolfes RJ.
J Biol Chem. 2016 Mar 25;291(13):6772-83. doi: 10.1074/jbc.M116.714907. Epub 2016 Jan 31.
PMID: 26828065 Free PMC Article
The GRF10 homeobox gene regulates filamentous growth in the human fungal pathogen Candida albicans.Ghosh AK, Wangsanut T, Fonzi WA, Rolfes RJ.
FEMS Yeast Res. 2015 Dec;15(8). pii: fov093. doi: 10.1093/femsyr/fov093. Epub 2015 Oct 15.
PMID: 26472755 Free PMC Article
Using electrospun poly(ethylene-oxide) nanofibers for improved retention and efficacy of bacteriolytic antibiotics.
Gatti JW, Smithgall MC, Paranjape SM, Rolfes RJ, Paranjape M.
Biomed Microdevices. 2013 Oct;15(5):887-93. doi: 10.1007/s10544-013-9777-5.
PMID: 23764950 [PubMed - in process]
Candida albicans isolates from the gut of critically ill patients respond to phosphate limitation by expressing filaments and a lethal phenotype.
Romanowski K, Zaborin A, Valuckaite V, Rolfes RJ, Babrowski T, Bethel C, Olivas A, Zaborina O, Alverdy JC.
PLoS One. 2012;7(1):e30119. doi: 10.1371/journal.pone.0030119. Epub 2012 Jan 13.
PMID: 22253901 [PubMed - indexed for MEDLINE] Free PMC Article
Goa1p of Candida albicans localizes to the mitochondria during stress and is required for mitochondrial function and virulence.
Bambach A, Fernandes MP, Ghosh A, Kruppa M, Alex D, Li D, Fonzi WA, Chauhan N, Sun N, Agrellos OA, Vercesi AE, Rolfes RJ, Calderone R.
Eukaryot Cell. 2009 Nov;8(11):1706-20. doi: 10.1128/EC.00066-09. Epub 2009 Aug 28.
PMID: 19717740 [PubMed - indexed for MEDLINE] Free PMC Article
Activation of the ADE genes requires the chromatin remodeling complexes SAGA and SWI/SNF.
Koehler RN, Rachfall N, Rolfes RJ.
Eukaryot Cell. 2007 Aug;6(8):1474-85. Epub 2007 Jun 15.
PMID: 17573544 [PubMed - indexed for MEDLINE] Free PMC Article
Regulation of purine nucleotide biosynthesis: in yeast and beyond.
Rolfes RJ.
Biochem Soc Trans. 2006 Nov;34(Pt 5):786-90.
PMID: 17052198 [PubMed - indexed for MEDLINE]
DNA-bound Bas1 recruits Pho2 to activate ADE genes in Saccharomyces cerevisiae.
Som I, Mitsch RN, Urbanowski JL, Rolfes RJ.
Eukaryot Cell. 2005 Oct;4(10):1725-35.
PMID: 16215179 [PubMed - indexed for MEDLINE] Free PMC Article
Mutations in the Pho2 (Bas2) transcription factor that differentially affect activation with its partner proteins Bas1, Pho4, and Swi5.
Bhoite LT, Allen JM, Garcia E, Thomas LR, Gregory ID, Voth WP, Whelihan K, Rolfes RJ, Stillman DJ.
J Biol Chem. 2002 Oct 4;277(40):37612-8. Epub 2002 Jul 26.PMID: 12145299 [PubMed - indexed for MEDLINE] Free Article
Functional mapping of Bas2. Identification of activation and Bas1-interaction domains.
Hannum C, Kulaeva OI, Sun H, Urbanowski JL, Wendus A, Stillman DJ, Rolfes RJ.
J Biol Chem. 2002 Sep 13;277(37):34003-9. Epub 2002 Jul 10.
PMID: 12110691 [PubMed - indexed for MEDLINE] Free Article
ade9 is an allele of SER1 and plays an indirect role in purine biosynthesis.
Buc PS, Rolfes RJ.
Yeast. 1999 Sep 30;15(13):1347-55.
PMID: 10509016 [PubMed - indexed for MEDLINE]
